Purification of the G-protein G13 from rat brain membranes
نویسندگان
چکیده
منابع مشابه
Practical Techniques for Improving the Performance of Polymeric Membranes and Processes for Protein Separation and Purification
Protein separation and purification technologies play an essential role in various industries including but not limited to pharmaceuticals, dairy as well as the food sector. Accordingly, a wide variety of techniques such as chromatography and electrophoresis has been developed and utilized extensively over the years for this purpose. Despite their widespread acceptance, conventiona...
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A method is described for the preparation of a B-galactosidase which acts on the brain lipid, ceramide galactoside. The enzyme is obtained from particles that sediment between I 000-13 ooo xg from rat brains and is extracted with a 60,& sodium cholate solution. The enzyme is solubilized and inactive proteins removed by digestion with pancreatic enzymes and the cholate is removed by dialysis and...
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A1 adenosine receptors from rat brain membranes were solubilized with the zwitterionic detergent 3-[3-( cholamidopropyl)dimeth ylammonio ]-1-propanesulfonate. The solubilized receptors retained all the characteristics of membrane-bound A1 adenosine receptors. A high and a low agonist affinity state for the radiolabelled agonist (R)-N'-eHJphenylisopropyladenosine((JH]PJA) with Kn values of 0.3 a...
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The present paper describes the characterization of the Thy-1 molecule from rat brain. The molecule was recognized by its antigens, which could be solubilized from brain membrane with deoxycholate. In the solubilized form the Thy-1 antigens were associated with a homogenous component with the following hydrodynamic properties: s20,w=2.2s,v=0.72ml/g and Stokes radius=3.0nm. The mol.wt. of the de...
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In continuing studies in this laboratory on the role of thiamine in nervous tissue we have recently accumulated some evidence to suggest that the neurophysiologically active form of the vitamin may be thiamine triphosphate (TTP) as opposed to the coenzyme form, thiamine pyrophosphate (thiamine-PP) (l-3). Since a specific enzyme exists in brain which catalyzes the hydrolysis of thiamine-PP (4), ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1994
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3030135